MADELINE G. EPPLEY
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Field Stories: Chesapeake Bay, Virginia

12/6/2024

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By Madeline Eppley, Zea Segnitz, and Zoe Chapman

The Chesapeake Bay is one of the largest estuaries in the world, known for its historic, cultural, and ecological significance. Recently, we embarked on a fieldwork trip to the Virginia Institute of Marine Science to study one of the most iconic species of the Chesapeake Bay, the eastern oyster (C. virginica). Our research contributes insight towards understanding how oyster genetics impact survival and success under different environmental conditions.

​While there, our main goal was to monitor success of oysters growing in an ongoing
 experiment for the Lotterhos lab. This experiment involves raising juvenile eastern oysters with a variety of genetic backgrounds in two different sites within the Chesapeake Bay, the Lewisetta River and the York River. Our visit marked the 18-month point in the study, so we could really start to see substantial differences in survival and success across groups!  
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So, what does day-to-day field work look like for marine biologists? As it turns out, there really isn't one standard day! We're going to walk you through each of our days as marine biologists working on oyster genetics in the Chesapeake Bay. While we are in the field, we do everything from boating to sampling sites, working in the field lab, collaborating with other researchers at VIMS, and spending lots of time outside! 

8:00 am: Our first task of the day is to drive two hours north to the Lewisetta River in Maryland, where one of our study sites is! We raise the oysters in mesh bags on racks at each site, which allows us to sample them twice per year for growth and mortality. 

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​10:00 am:
Once we collected our samples, we return to the Aquaculture Genetics & Breeding Technology Center in the Acuff Center for Aquaculture at the Virginia Institute of Marine Science. Zea works on collecting samples for her own independent thesis! Madeline removes the predatory blue crabs from bags, where they have been eating the oysters. Unfortunately, this predation pressure can result in loss of study samples if the crabs are not routinely removed from the bags. 
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​12:00 pm: We enjoy the beautiful November weather in Virginia by eating some lunch outside. We are close enough to the York River on the VIMS campus that we can see it from our field lab, where we have been working on collecting data this morning!
1:00 pm: Our afternoon starts by driving the tractor to the York River, where our second group of oyster bags are located. In our study, each of the two sites has a distinct environment. For example, the Lewisetta River is a low-salinity site, and the York River is a high-salinity site! 

When we arrive at the site, Zoe pulls up metal cages that are sitting on the bottom of the river bed using a large hook. These cages are long lines, meaning they're all attached to a rope underwater. They cages are heavy, so it takes effort to pull them up! 

Inside of each metal cage, there are three mesh bags that hold the oysters in the study. We make sure to collect the samples at low tide so that the water level is low enough for us to go out in our waders!
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3:00 pm: Once we pull the oyster bags out of the water, we're under a time crunch to process data. Eastern oysters are filter feeders, meaning they need water to breathe, eat, and maintain basic function. They become stressed outside of the water, so we process them quickly and return them within 48 hours to the river. These oysters will continue to live over the next winter and we will check on them again in May 2025!

​5:00 pm: An important part of doing rigorous science is checking your work! At the end of our day, Madeline reviews all of the data that we collected. We check for errors and completeness. We also make an entry in our log, which is like a journal for our field data. This helps us to keep track of the environmental conditions at the site each day, hours worked, and any other important information.
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Curious what else we do in a day at VIMS? Check out our video to see more clips and highlights from a day in the life as a marine biologist in the Chesapeake Bay! 

Undergraduate researchers Zea Segnitz 
(zeasegnitz.bsky.social) and Zoe Chapman 
​(zoechapman.bsky.social) are continuing on in the Lotterhos lab with independent projects slated for 2025/2026, stay tuned for the results of their work!
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Field Stories: Coastal Resiliency in Georgia

8/26/2024

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On a recent trip to Georgia, I found myself unexpectedly (and directly!) in the path of Tropical Storm Debby. The rainfall, flooding, and wind prevented me from visiting Sapelo Island, but I was still able to participate in a week long workshop about science communication and bioinformatics in Savannah with some fellow marine scientists.  
While we experienced substantial rainfall in Savannah for several days, there was less flooding than initially forecasted. Given the circumstances, I was curious about the factors of coastal resiliency in Georgia that may have contributed to reducing the impact of flooding during Debby.

​Having collected wild oysters from Georgia for my dissertation, I knew that there are expansive natural oyster reef structures along the coastlines in the greater Savannah area. Specifically, I know that native oyster reefs play a role in shielding the coast from storm surge and erosion. 
​
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Tropical Storm Debby was forecasted to bring close to 30 inches of rain to Savannah, GA, a record rainfall. Image credit: NOAA & Savannah Morning News
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Some wild local oysters near Savannah, GA. Some oyster reefs occur deeper in the water column (subtidally) where they are fully covered by water. This reef is intertidal, where the oysters are exposed to air during low tides.
With a growing number of people living in coastal areas along the Atlantic and Gulf coasts of the US, it's imperative to better understand coastal mitigation strategies in the face of rapidly intensifying storms and climate change events. Natural oyster reefs are ecosystem engineers that provide a physical barrier which mitigates storm surges and prevents successive erosion (Chowdhury et al 2021). Beyond physical factors, filter-feeding oysters clear bacteria that enters the water through storm drainage and runoff. Oyster reefs also provide valuable structural habitat for many marine species. This habitat is the foundation for biodiverse ecosystems and healthy fisheries that remain resilient to extreme climatic events (Chowdhury et al 2021). ​
​To investigate additional factors of coastal resilience in Georgia, I visited Tybee Island, just east of Savannah, with other workshop attendees. While there, we were inundated with heavy rain and wind, but we were able to identify several features of the landscape that, in part, play a role in buffering the coastline from storms. 

​I teamed up with scientists Damián Santiago-Sosa, Paul Okrah, Darrian Talamantes, and Mai Fahmy, who shared their expertise on resilient coastal ecosystems and how marine scientists study these coastlines in the video that we created!

Check out our video on features of resilient coastlines such as sand dunes, bacteria, and biodiversity! These resilient features are similar among many coastlines in different marine environments, so next time you're on the coast, see how many of these features you can identify.
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We encountered strong winds & rain on Tybee Island from Tropical Storm Debby!
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Coastal scientists Paul Okrah and Damián Santiago-Sosa on Tybee Island with me!
Citations
1. Chowdhury, Mohammed Shah Nawaz, Megan La Peyre, Loren D. Coen, Rebecca L. Morris, Mark W. Luckenbach, Tom Ysebaert, Brenda Walles, and Aad C. Smaal. "Ecological engineering with oysters enhances coastal resilience efforts." Ecological Engineering 169 (2021): 106320​
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Protocol: ImageJ for Oyster Shell Parasites

7/18/2024

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Few studies have investigated how abiotic, biotic, and genetic conditions combined impact range-wide infestation rates of parasites on wild eastern oysters. I aim to map these spatial patterns of parasite infestation across the seascape and determine whether patterns correlate with environmental factors (temperature, salinity, co-occurring parasitic infestations) and/or population genetics. To achieve this, I am mapping the prevalence of parasitic Polydora worm blisters in eastern oyster shells.

​I've co-authored a lab protocol for using ImageJ to extract parasitic worm blister data with Lotterhos Lab summer intern Lisa Gouralnik! This protocol provides guidelines for identifying polydora worm blisters, using ImageJ to calculate total shell areas and areas of blister infection, and creating high-quality composite images. 
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The Polydora worm burrows into oyster shells, creating irritation and weakening shell integrity. Infected oysters exhibit a lower condition index, a measure of health, than uninfected oysters.
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Eastern oyster shells from the coastal Atlantic and Gulf of Mexico show parasitic worm blisters, outlined in dashed lines.
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Above: Lisa in the shell imaging lab at the Marine Science Center. Right: Excerpt from the protocol about collecting data on complex colored blisters.
Lisa collected >2000 data points on the polydora worm blisters over the past two months of their internship. It was great to mentor them on this project, and I'm excited to get to the next steps of data analysis!
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Melbourne R. Carriker Research Award

4/8/2024

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Last week I was awarded the Melbourne R. Carriker Student Research Grant from the National Shellfisheries Association! The award is a competitive grant ($1250) awarded annually to recognize a student's excellence in the area of shellfish research. I am grateful to the NSA's funding and support towards my research, and I look forward to presenting my research at an upcoming NSA meeting.
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​The Melbourne R. Carriker Student Research Grant is named in honor of one of the Association's most distinguished past Presidents and the person who formalized the regular publication of the society meeting notes as the Proceedings of the National Shellfisheries Association.
My successful application materials can be accessed through the "Awards and Grants" section of my website! If you are interested in applying for the award in future cycles and would like to get in touch, please send me an email. 
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Copyright Madeline Eppley, 2025

LAND ACKNOWLEDGEMENT
We acknowledge the territory on which Northeastern University’s Marine Science Center stands, which is the land of the Mattakeeset tribe (1) of the Massachuset Nation (2,5) and which has been inhabited by the Pawtucket (3,5) and Naumkeag (4,5) people. We honor and respect these peoples' past, present, and future, their continuing presence in this region, and the enduring relationships that exist between them and these lands. We strive to be mindful of these relationships, and to integrate them into our research, teaching, decision-making, and actions, while also acknowledging that we still have much to learn.   

​Citations:  
1.      
https://www.mattakeeset.com/history   
2.      
https://accessgenealogy.com/massachusetts/massachuset-tribe.htm   
3.      
https://capeannhistory.org/index.php/chapter-5-what-native-people-were-on-cape-ann-at-the-time-of-contact-and-where-did-they-come-from/   
4.      
http://www.salemhistorical.org/massachusetts-indigenous-community-resources
​5.       https://native-land.ca/ 
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